Bilberry extract, antioxidant activity of ANTHO 50®

ANTIOXIDANT ACTIVITY OF ANTHO 50® IN RATS (3)

Experimental Design



• Anthocyanins are involved in multiple biological activities such as vasoprotective effects, anti-inflammatory action, antiatherosclerotic and anticarcinogenic activities.
The positive effects of anthocyanins could be attributable to their potent antioxidant activity. However, little is known about their potential antioxidant capacity in vivo.

• The purified extract of bilberry (Antho50®, Ferlux Mediolanum, Cournon d’Auvergne, France) contains fifteen different anthocyanins eluted as thirteen peaks (Fig 1). Anthocyanins are 3-O glycosides :
(glucoside: glc, arabinoside: ara, and galactoside: gal) of five aglycons (cyanidin: Cy; delphinidin: Dp; peonidin: Pn; petunidin: Pt; malvidin: Mv).

Plasma antioxidant capacity



• Plasma antioxidant capacity determined by the FRAP assay was significantly increased by the bilberry extract enrichied diet (* p<0.05) at both 3 and 6h after the beginning of the last meal.

• This effect could probably be attributed to anthocyanin metabolites or degradation products, because we failed to detect anthocyanins in plasma.

Conclusion :

Bilberry anthocyanin consumption has a positive effect on plasma antioxidant capacity.